What Does EEM Stand For?

EEM stands for Excitation Emission Matrix

An Excitation Emission Matrix (EEM) is a 3D representation of fluorescence data, widely used in spectroscopy, particularly for analysing complex mixtures of fluorophores. It combines the fluorescence emission spectra obtained at various excitation wavelengths into a matrix, providing a comprehensive fingerprint of the sample's fluorescent properties. Key Components: 1. Excitation Wavelength (x-axis): The wavelength of light used to excite the sample. 2. Emission Wavelength (y-axis): The wavelength of the fluorescence emitted by the sample after excitation. 3. Fluorescence Intensity (z-axis): The magnitude of fluorescence measured for each pair of excitation and emission wavelengths. Visualization: An EEM is typically represented as a 2D heatmap or contour plot: - x-axis: Excitation wavelength. - y-axis: Emission wavelength. - Color or intensity values: Correspond to fluorescence intensity at each excitation-emission pair. Applications: - Environmental Science: Identifying organic matter, pollutants, or fluorescent tracers in water. - Biology: Studying protein folding, enzyme activities, or the behaviour of fluorophores in biomolecules. - Chemical Analysis: Characterizing fluorescent compounds, including dyes, drugs, or polymers. - Food Science: Quality control and detection of contaminants. Benefits: - Comprehensive data: Captures the complete fluorescence behaviour of a sample. - Fingerprinting capability: Helps identify and distinguish between different fluorophores in a mixture. Example: If a sample contains multiple fluorophores, each with a unique fluorescence profile, the EEM will show distinct peaks corresponding to the excitation-emission pairs of those fluorophores. These peaks can then be analyzed to identify or quantify the components of the mixture.

Category : Scientific

EEM is an Initialism

Other Meanings for EEM